重组鸡GSTA3蛋白对福美双诱导胫骨软骨发育不良肉鸡红细胞免疫相关基因转录的影响

doi:10.11843/j.issn.0366-6964.2018.04.019

Abstract

Abstract:

The objective of this study was to evaluate the transcriptional changes of immune related genes and the effection of recombinant chicken glutathione S-transferase A3 (chGSTA3) protein on the transcription of immune related genes in erythrocytes of Tibial Dyschondroplasia (TD) broiler chickens. Therefore, the model of TD broiler chickens was established, in which 120 broiler chickens were randomly broken up into 6 groups; group A, B and C (fed with basal diet), group D, E and F (fed with basal diet containing 100 mg·kg^-1 Thiram) after one week normal feeding. The broiler chickens of group B and E were treated with chGSTA3 protein by intramuscular injection (low dose:20 μg·kg^-1) and the broilers of group C and F were treated intra-muscularly with chGSTA3 protein (high dose:50 μg·kg^-1), while groups A and D received the same dosage of phosphate buffered solution (PBS). We used Real-time PCR to investigate the mRNA expression of the immune-related genes in chicken erythrocytes. The transcripts for TLR2, TLR3, TLR4, TLR5, TLR7, TLR15, myeloid differentiation factor 88 (MyD88), major histocompatibility complex (MHC) class Ⅱ, Nucleotidebinding oligomerization domain receptor caspase recruitment domain 5 (NLRC5), TNF receptor-associated factor 6 (TRAF6) and Interleukin (IL-7) were constitutively expressed in erythrocytes of healthy broiler chickens. Contrasted with group A with phosphate buffered solution (PBS) treatment, the mRNA transcription level of TLR2, TLR3, TLR4, TLR5, TLR7, TLR15, MyD88, MHCⅡ, NLRC5, TRAF6 of Thiram-treated chicken erythrocytes in group D on the 4^th day were significantly upregulated (P<0.05), whereas IL-7 was significantly downregulated. We observed that the transcripts of immune-related genes:TLR2, TLR4, MyD88 and NLRC5 in the chicken erythrocytes of group D were significantly downregulated than that in group A (P<0.05), but IL-7, TLR3, TLR5, TLR7, TLR15, MHCⅡ and TRAF6 were insignificant (P>0.05) on the 15^th day. The changes of above mentioned genes mRNA expression in group E and F were significant except TLR4, TLR5, MyD88 in group E as compared with group D on the 4^th day. The changes of TLR4, TLR5, MyD88, NLRC5 genes in group E and TLR3, TLR5, MyD88, TRAF6, IL-7 genes in group F were insignificant, whereas the others were significant compared with group D on the 15^th day. Broiler chicken erythrocytes could induce the occurrence of TD in the early stage and alleviate TD symptoms in the late stage by changing the mRNA expression of IL-7, TLR2, TLR3, TLR4, TLR5, TLR7, TLR15, MyD88, MHCⅡ, NLRC5, TRAF6. The recombinant chicken glutathione S-transferase A3 protein could influence TD development caused by Thiram.

CLC Number:

S852.34

JIA Fa-jie, NIU Sheng, ZHANG Ning, LI Xin, NING Guan-bao, ZHANG Ding, LI Hong-quan, MA Hai-li, HAO Wei-fang, GAO Wen-wei, ZHAO Yu-jun, GAO Shi-min, LI Gui-lan, LI Jian-hui, YAN Fang, GAO Rong-kun, TIAN Wen-xia. The Effection of Recombinant Protein GSTA3 on the Transcription of Erythrocytes Immune Related Genes in Tibial Dyschondroplasia Broilers Induced by Thiram[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 811-817.

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The Effection of Recombinant Protein GSTA3 on the Transcription of Erythrocytes Immune Related Genes in Tibial Dyschondroplasia Broilers Induced by Thiram

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